Nemecz Lab

Aperçu de la Recherche

Le laboratoire fait partie du département de biochimie de l'université Nicolas Copernic de Torun, en Pologne. Nous nous concentrons sur l'étude des mécanismes moléculaires impliquant la fonction et la régulation des récepteurs canaux pentamérique (pLGICs). La famille des pLGIC, chez les mammifères, comprend les excitateurs : récepteurs nicotiniques de l'acétylcholine (nAChRs), les récepteurs de la sérotonine de type 3 (5-HT₃Rs) et les canaux ioniques activés par le zinc, et les inhibiteurs : les récepteurs de l'acide γ-aminobutyrique de type A (GABA_ARs) et les récepteurs de la glycine. (GlyRs). La famille pLGIC relaie la transduction de signalisation chimique-électrique des neurones. La liaison des neurotransmetteurs synaptiques libérés stabilise l'ouverture d'un canal intrinsèque conduisant passivement les ions à travers la membrane, ce qui déclenche ainsi la dépolarisation ou l'hyperpolarisation cellulaire. Comme ils sont exprimés de manière ubiquitaire dans pratiquement tous les neurones, les pLGIC contribuent à toutes les fonctions majeures du système nerveux central; y compris : traitement sensoriel et moteur, contrôle autonome central, mémoire et attention, sommeil et éveil, récompense, douleur, anxiété, émotions et cognition. La toxicomanie, en particulier le tabagisme, et les désordres neurologiques tels que la maladie d'Alzheimer, la schizophrénie et la maladie de Parkinson sont depuis longtemps liés au système cholinergique, en particulier aux nAChRs. Malheureusement, les nombreuses années d'études pharmacologiques n'ont pas abouti à des thérapies robustes pour les malédictions causées par un dysfonctionnement des nAChRs qui assaillent les individus. Et donc, en utilisant de nombreuses facettes des techniques biochimiques, de la biologie moléculaire, de la biochimie des protéines, de l'électrophysiologie, de l'immunofluorescence et de la pharmacologie, notre objectif est de faire briller des informations sur le domaine intracellulaire et donc la régulation/le trafic de ces récepteurs. Cibler leurs protéines régulatrices peut mieux combattre les troubles neurodégénératifs que cibler les récepteurs eux-mêmes.

Membres de l'Équipe

Nous sommes toujours à la recherche de personnes motivées et intéressées par nos recherches. Si vous vous sentez qualifié, contactez-nous.

adiunkt

Dr. Ákos Nemecz

adiunkt

dr Dorota Nemecz

Mascotte/Gardienne du Labo

Onyx

Postdoctorants

Doctorants

Étudiants en Master

Biotechnologie

Weronika Nowak

Biotechnologie

Katarzyna Świstowska

Biotechnologie

Zuzanna Szutkowska

Étudiants en Licence

Biotechnologie

Kamila Grygiel

Biotechnologie

Marcelina Mikołajczyk

Biotechnologie

Magda Tylenda

Biotechnologie

Kuba Wolinski

Anciens membres

Étudiants en Licence

Agata
Wikarska

Étudiants en Licence

Kacper
Roszak

Étudiants en Licence

Jakub
Lewandowski

Projets

Enquête sur le mécanisme de régulation des récepteurs nicotiniques de l'acétylcholine
POLS-2020/37/K/NZ3/04098

Combattre l'addiction en ciblant les récepteurs α3β4*-nicotiniques de l'acétylcholine
OPUS21-2021/41/B/NZ7/03101

L'actualités du Labo

• Nous recherchons un(e) étudiant(e) exceptionnel(le) pour un poste de doctorant rémunéré:
  Doctoral Student w/ Stipend Announcement within NCN OPUS-21 Project
  Wydział Nauk Biologicznych i Weterynaryjnych/Faculty of Biological and Veterinary Sciences
  Uniwersytet Mikołaja Kopernika w Toruniu/Nicolaus Copernicus University in Toruń
  
  Start Date: April 1st 2024
  Stipend Level: 5,000 PLN/month (gross/gross [brutto/brutto]: employer taxes, pension, health insurance and income taxes are deducted from this amount
  Stipend Duration: 48 months
  
  Project Description: Generation and characterization of synthetic single domain VHH antibodies against nicotinic acetylcholine receptor subtypes. Under the supervision of the project leader: Dr. Ákos Nemecz. More information about the lab and projects may be found at: nemeczlab.umk.pl
  
  Requirements:
  

  • Master of Science in biochemistry, pharmacology, biology, or related field, completed before beginning of doctoral dissertation.
    
  • Experience in a biochemical laboratory
  • Good communication skills.
  • Proficiency in written and spoken English.
  
  

  Please send the following application materials to Dr. Ákos Nemecz:
  

  • A cover/motivation letter
  • CV
  • A list of contact information (name, email, position/institution) for two references included in either the CV or cover letter
  • A signed GDPR statement: Found here
• Le Nemecz Lab a un poste de postdoctoral ouvert:
  Post-Doctoral Position Announcement within NCN OPUS-21 Project
  Uniwersytet Mikołaja Kopernika w Toruniu/Nicolaus Copernicus University in Toruń
  
  Start Date: As soon as possible
  Funding: 10,000 PLN/month (gross/gross: employer taxes, pension, health insurance and income taxes are deducted from this amount ≈7608 PLN/month [gross: personal income tax and social security deducted from this amount] with 13th month)
  Duration: 24month initial contract with possibility to extend an additional year.
  
  Candidatures will be evaluated on a rolling basis, until the position is filled.
  
  Project Description: Generation and characterization of synthetic single domain VHH antibodies against nicotinic acetylcholine receptor subtypes. Localization of these subtypes in the brain tissue samples. Under the supervision of the project leader: Dr. Ákos Nemecz. More information about the lab and projects may be found at: nemeczlab.umk.pl
  
  Requirements:
  

  • Ph.D. in biochemistry, pharmacology, biology, or related field.
    (PhD degree must be awarded by another institution than UMK w Toruniu or a continuous post-doctoral fellowship of at least 10 months has been completed, in another country than Poland, prior to hire);
    
  • Ability to work independently on a scientific project including: planning, completing, analyzing experiments as well as the ability to properly present the results;
  • Fluent in written and spoken English.
  
  

  Strongly preferred:
  

  • Experience in protein purification techniques and immunostaining
  • Good communication and team working skills
  
  

  Please send the following application materials to Dr. Ákos Nemecz:
  

  • A cover/motivation letter
  • CV, including list of publications/abstracts
  • A list of contact information (name, email, position/institution) for three references included in either the CV or cover letter
  • A signed GDPR statement: Found here
• 2023-02-20: dr Dorota Nemecz présentera son abstract lors de la réunion annuelle de la Biophysical Society à San Diego. Venez nous voir @13:45 Exhibit Hall BC Poster#:LB75.
• 2022-10-27: Séminaire départemental par Dr. Ákos Nemecz à 10h salle C226
• Le labo a remporté l'une des quatre places du concours Emerging Fields 2022 dans le cadre de la groupe collaboratif: Cells as EXperimental platforms and bioFACTories (CExFact) dirigé par le dr hab. Krzysztof Zienkiewicz. Voir l'annonce ici (en polonais):
• 2022-06-10: Dans le cadre du projet POWER “Universitas Copernicana Thoruniensis in Futuro - modernisation de l'Université Nicolaus Copernicus dans le cadre du Programme Universitaire Intégré” Dr. Pierre-Jean Corringer de l'Institut Pasteur donnera un seminaire: “Illuminating the gating transition of pentameric channel-receptors”
  Le 10 Juin 2022, 11:00h dans la Salle Darwin Pub

Publications

2024

• Van Renterghem C.^§, Nemecz Á., Medjebeur K., Corringer P-J.^§ “Short-chain mono-carboxylates as negative modulators of allosteric transitions in Gloeobacter violaceus ligand-gated ion channel, and impact of a pre-β5 strand (Loop Ω) double mutation on crotonate, not butyrate effect.” Physiol Rep (2024-02-11); 12(3):e15916. DOI: 10.14814/phy2.15916

2023

• Van Renterghem C.^§, Nemecz Á., Delarue-Cochin S., Joseph D., Corringer P-J.^§ “Fumarate as positive modulator of allosteric transitions in the pentameric ligand-gated ion channel GLIC: Requirement of an intact vestibular pocket.” J Physiol (2023-06); 601(12):2447-2472. DOI: 10.1113/JP283765. Epub 2023-04-27
• Li Q., Nemecz Á.^§, Aymé G.^§, Dejean de la Bâtie G., Prevost M.S., Pons S., Barilone N., Baachaoui R., Maskos U., Lafaye P., Corringer P-J.^§ “Generation of nanobodies acting as silent and positive allosteric modulators of the α7 nicotinic acetylcholine receptor.” Cell Mol Life Sci (2023-05-25); 80(6):164. DOI: 10.1007/s00018-023-04779-8

2022

• Nemecz D.^§, Golińska P. “Antibacterial Properties of Crotoxin from Crotalus durissus terrificus-Insight into the Mechanism of Action.” Molecules (2022-11-10); 27(22):7726. DOI: 10.3390/molecules27227726

2020

• Nemecz D.*, Ostrowski M.*, Ravatin M., Saul F., Faure G.^§ “Crystal Structure of Isoform CBd of the Basic Phospholipase A2 Subunit of Crotoxin: Description of the Structural Framework of CB for Interaction with Protein Targets.” Molecules (2020-11-13); 25(22):5290. DOI: 10.3390/molecules25225290

2018

• Hu H.*, Nemecz Á.*, Fourati Z., Van Renterghem C., Sauguet L., Corringer P-J., Delarue M.^§ “The active form of a new bacterial pentameric ligand-gated ion channel displays a widely open pore and is stabilized by an allosteric activator.” Proc Natl Acad Sci U S A (2018-04-24); 115(17):E3959-E3968. DOI: 10.1073/pnas.1717700115
• Bajek A., Olkowska J., Walentowicz-Sadłecka M., Sadłecki P., Grabiec M., Porowińska D., Drewa T., Roszkowski K.^§ “Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability.” Med Sci Monit. (2018-03-24); 24:1733-1741. DOI: 10.12659/msm.905826

2017

• Nemecz Á., Hu H., Fourati Z., Van Renterghem C., Delarue M., Corringer P-J.^§ “Full mutational mapping of titratable residues helps to identify proton-sensors involved in the control of channel gating in the Gloeobacter violaceus pentameric ligand-gated ion channel.” PLoS Biol (2017-12-27); 15(12):e2004470. DOI: 10.1371/journal.pbio.2004470
• Maj M.*^§, Bajek A.*, Nalejska E., Porowińska D., Kloskowski T., Gackowska L., Drewa T. “Influence of Mesenchymal Stem Cells Conditioned Media on Proliferation of Urinary Tract Cancer Cell Lines and Their Sensitivity to Ciprofloxacin.” J Cell Biochem. (2017-06); 118(6):1361-1368. DOI: 10.1002/jcb.25794. Epub 2016-11-30.
• Wujak M., Hetmann A., Porowińska D., Roszek K.^§ “Gene Expression and Activity Profiling Reveal a Significant Contribution of Exo-Phosphotransferases to the Extracellular Nucleotides Metabolism in HUVEC Endothelial Cells.” J Cell Biochem. (2017-06); 118(6):1341-1348. DOI: 10.1002/jcb.25791. Epub 2017-01-03.
• Czarnecka J., Porowińska D., Bajek A., Hołysz M., Roszek K.^§ “Neurogenic Differentiation of Mesenchymal Stem Cells Induces Alterations in Extracellular Nucleotides Metabolism.” J Cell Biochem. (2017-03); 118(3):478-486. DOI: 10.1002/jcb.25664. Epub 2016-08-25.
• Bajek A.^§, Olkowska J., Walentowicz-Sadłecka M., Walentowicz P., Sadłecki P., Grabiec M., Bodnar M., Marszałek A., Dębski R., Porowińska D., Czarnecka J., Kaźmierski Ł., Drewa T. “High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells-A Practical Analysis Based on 165 Clinical Cases.” J Cell Biochem. (2017-01); 118(1):116-126. DOI: 10.1002/jcb.25618. Epub 2016-06-28.
• Roszek K.^§, Makowska N., Czarnecka J., Porowińska D., Dąbrowski M., Danielewska J., Nowak W. “Canine Adipose-Derived Stem Cells: Purinergic Characterization and Neurogenic Potential for Therapeutic Applications.” J Cell Biochem. (2017-01); 118(1):58-65. DOI: 10.1002/jcb.25610. Epub 2016-06-03.

2016

• Ostrowski M.^§, Mierek-Adamska A., Porowińska D., Goc A., Jakubowska A. “Cloning and biochemical characterization of indole-3-acetic acid-amino acid synthetase PsGH3 from pea.” Plant Physiol Biochem. (2016-10); 107:9-20. DOI: 10.1016/j.plaphy.2016.05.031. Epub 2016-05-20.
• Ostrowski M., Porowińska D., Prochnicki T., Prevost M., Raynal B., Baron B., Sauguet L., Corringer P-J., Faure G.^§ “Neurotoxic phospholipase A2 from rattlesnake as a new ligand and new regulator of prokaryotic receptor GLIC (proton-gated ion channel from G. violaceus).” Toxicon (2016-06-15); 116:63-71. DOI: 10.1016/j.toxicon.2016.02.002. Epub 2016-02-05.
• Nemecz Á.*, Prevost, M.S.*, Menny A.*, Corringer P-J.^§ “Emerging Molecular Mechanisms of Signal Transduction in Pentameric Ligand-Gated Ion Channels.” Neuron (2016-05-04); 90(3):452-70. Review. DOI: 10.1016/j.neuron.2016.03.032

2015

• Roszek K.^§, Porowińska D., Bajek A., Hołysz M., Czarnecka J. “Chondrogenic Differentiation of Human Mesenchymal Stem Cells Results in Substantial Changes of Ecto-Nucleotides Metabolism.” J Cell Biochem. (2015-12); 116(12):2915-23. DOI: 10.1002/jcb.25239
• Bajek A.*^§, Porowińska D.*, Kloskowski T., Brzoska E., Ciemerych MA., Drewa T. “Cell therapy in Duchenne muscular dystrophy treatment: clinical trials overview.” Crit Rev Eukaryot Gene Expr. (2015); 25(1):1-11. DOI: 10.1615/critreveukaryotgeneexpr.2015011074

2014

• Stadnicka K., Marszałek A., Kozłowska I., Walasik K., Bodnar M., Bajek A., Porowińska D., Drewa T., Bednarczyk M. “The expression of p63 and Ck HMW in magnum and infundibulum of Gallus domesticus oviduct.” Folia Biol (Krakow) (2014); 62(3):179-85. DOI: 10.3409/fb62_3.179
• Sauguet L.*, Shahsavar A.*, Poitevin F.*, Huon C., Menny A., Nemecz Á., Haouz A., Changeux J-P, Corringer P-J, Delarue M.^§ “Crystal structures of a pentameric ligand-gated ion channel provide a mechanism for activation.” Proc Natl Acad Sci U S A (2014-01-21); 111(3):966-71. DOI: 10.1073/pnas.1314997111

2013

• Uzarska M.^§, Porowińska D., Bajek A., Drewa T. “[Epidermal stem cells--biology and potential applications in regenerative medicine].” Postepy Biochem. (2013); 59(2):219-27.
• Wujak M., Banach M., Porowińska D., Piskulak K., Komoszyński M.^§ “Isolation and bioinformatic analysis of seven genes encoding potato apyrase. Bacterial overexpresssion, refolding and initial kinetic studies on some recombinant potato apyrases.” Phytochemistry (2013-09); 93:8-17. DOI: 10.1016/j.phytochem.2013.03.014. Epub 2013-05-09.
• Porowińska D.^§, Wujak M., Roszek K., Komoszyński M. “[Prokaryotic expression systems].” Postepy Hig Med Dosw (Online) (2013-03-01); 67:119-29. DOI: 10.5604/17322693.1038351

2012

• Yamauchi J.G., Gomez K., Grimster N.P., Dufoil M., Nemecz Á., Fotsing J.R, Ho K-Y., Talley T.T., Sharpless K.B., Fokin V.V, Taylor P.^§ “Synthesizing selective agonists for the α7 nicotinic receptor with in situ click-chemistry on acetylcholine binding protein templates.” Mol. Pharmacol. (2012-10); 82(4):687-99. DOI: 10.1124/mol.112.080291
• Rojsanga P., Boonyarat C., Utsintong M., Nemecz Á., Yamauchi J.G., Talley T.T., Olson A.J., Matsumoto K., Vajragupta O.^§ “The effect of crebanine on memory and cognition impairment via the alpha-7 nicotinic acetylcholine receptor.” Life Sci. (2012-08-21); 91(3-4):107-14. DOI: 10.1016/j.lfs.2012.06.017
• Porowińska D., Marszałek E., Wardęcka P., Komoszyński M.^§ “[In vitro renaturation of proteins from inclusion bodies].” Postepy Hig Med Dosw (Online) (2012-06-11); 66:322-9. DOI: 10.5604/17322693.999918
• Grimster N.P., Stump B., Fotsing J.R., Weide T., Talley T.T., Yamauchi J.G., Nemecz Á., Kim C., Ho K-Y., Sharpless K.B., Taylor P., Fokin, V.V.^§ “Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template.” J Am Chem Soc (2012-04-18); 134(15):6732-40. DOI: 10.1021/ja3001858

2011

• Nemecz Á., Taylor P.^§ “Creating an α7 nicotinic acetylcholine recognition domain from the acetylcholine binding protein: crystallographic and ligand selectivity analysis.” J Biol Chem (2011-12-09); 286(49):42555-65. DOI: 10.1074/jbc.M111.286583
• Yamauchi J.G., Nemecz Á., Nguyen Q.T., Muller A., Schroeder L.F., Talley T.T., Lindstrom J., Kleinfeld D., Taylor P.^§ “Characterizing ligand-gated ion channel receptors with genetically encoded Ca2++ sensors.” PLoS One (2011); 6(1):e16519. DOI: 10.1371/journal.pone.0016519
• Porowińska D., Czarnecka J., Komoszyński M.^§ “[The role of ectonucleotides metabolizing enzymes in purinergic signaling].” Postepy Biochem. (2011); 57(3):294-303.

Financement